In vitro regeneration of the endangered cactus Turbincarpus mombergeri (Riha), a hybrid of T. laui × T. pseudopectinatus

Turbinicarpus mombergeri is a cacti species formed by hybridization process between laui and pseudopectinatus. Under natural conditions, it very difficult for two be genetically compatible hybridization, to produce flowers at the same time. Thus, T. interesting rare species. Unfortunately, current populations are decreasing now considered critically endangered. The aim of this research was develop successful protocol propagating using in vitro culture techniques. Seed disinfection performed with Plant Preservative Mixture, 80% germination occurred day 45 Murashige-Skoog medium. shoots were cut longitudinally, segments transferred media containing 2.22 or 4.44 µM benzyladenine induce shooting. generated highly hydrated, presented abundant callus. hyperhydricity controlled reducing salt medium concentration, increasing calcium levels polyethylenglycol. reduction callus attained adding tri-iodo benzoic acid. Vigorous thick urea, rooting improved presence 0.5 indoleacetic Plantlets normal morphology obtained, survival rate plants soil 80%. methodology developed represents an alternative propagation under conditions commercial conservation purposes. paper describes several approaches avoid formation, improve quality increase development roots during micropropagation endangered mombergeri.